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OBJECTIVE:To study the antithrombotic effect of orientoside.METHODS:The effects of prophylactic oriento_side on the clotting time in vitro and the systemic clots dissolution of mice,the plasma prothrombin time(PT),the kaolin partial thromboplastin time(KPTT),the thrombin time(TT),and the platelet aggregation induced by ADP and the euglobulin lysis time (ELT)in rabbits were studied.RESULTS:As compared with the normal saline group,orientoside(1,2,and 4mg/kg)signi_ficantly prolonged the clotting time of mice in vitro,yet which had no significant influence on the systemic clots dissolution of mice;while orientoside(0.625,1.25 and 2.5 mg/kg)significantly prolonged the PT,KPTT and TT of rabbits,and significantly inhibited platelet aggregation of rabbits induced by ADP,yet which had no significant influence on the ELT of the rabbits.CONCLUSION:Orientoside can significantly inhibit the formation of thrombus.
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OBJECTIVE:To determine the content of chrysophanol in Diedazhitong cataplsam by HPLC.METHODS:The samples were separated on a Kromasasil C 18 ,methol-0.1%phosphoric acid(85∶15)was taken as the mobile phase with a flow rate at1.0ml/min and detection wavelength at254nm,the sample size was10?l.RESULTS:Good linear relationship was achieved when the concentration range of chrysophanol was0.6?g～4?g(r=0.9994);The average recovery of chrysophanol was99.23%(n=5,RSD=2.45%).CONCLUSION:This method is accurate,reliable,and reproducible.
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<p><b>OBJECTIVE</b>To observe triclabendazole effect on Paragonimus skrjabini in experimentally infected rats,and to develop a new drug for treating paragonimiasis.</p><p><b>METHODS</b>Metacercariae of Paragonimus skrjabini were isolated from crabs (Sinopotamon) collected from endemic area. Wistar rats were infected intraperitoneally. One and two months after infection, they were treated with triclabendazole at the dosage of 300 mg.kg(-1).2 d(-1), 450 mg.kg(-1).3 d(-1) and 600 mg.kg(-1).3 d(-1) respectively. Five patients with Paragonimus skrjabini were treated, with Triclabendazole dosage of 10 mg/kg bid x 3 days.</p><p><b>RESULTS</b>The worm reduction rates were 50.3%, 80.8% and 86.7% respectively one month after completion of treatment. Dead worms of sesame size recovered from muscles, liver, abdominal cavity, chest cavity and lung were greatly diminished in size and weight in comparison with that of the control group. Many large (about 1 cm) black-colored distended worm cysts were found in the lungs of the control rats. Usually there were two adult worms pairs with numerous eggs in each worm cyst. Most worm cysts in the treated groups of rats were changed into hemorrhagic-necrotic patches. All five patients were cured.</p><p><b>CONCLUSION</b>Triclabendazole was highly active against Paragonimus skrjabini in rats experimentally infected and patients.</p>
Subject(s)
Adult , Animals , Child , Female , Humans , Male , Rats , Anthelmintics , Therapeutic Uses , Benzimidazoles , Therapeutic Uses , Paragonimiasis , Drug Therapy , Parasite Egg Count , Rats, WistarABSTRACT
Objective To establish a method for the determination of arctiin in Pifu Zhiyang Tablets by SPE-HPLC . Methods SPE-HPLC method was performed on a Kromasil C18 column(150 mm?4.6 mm ,5 ?m),the mobile phase consisted of methanol -1 %acetic acid (35 ∶65) ,the wavelength was 283nm . Results The linear range of arctiin was 1.5～4.5 ?g(r= 0. 999 8) ,the average recovery was 98.18 %with RSD of 2.08 %. Conclusion This method is accurate ,reliable and reproducible. It can be used for the determination of arctiin in Pifu Zhiyang Tablets .
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Objective To study the effect of skin stratum corneum on transdermal absorption characteristic of Sinomenine Liposome Patch. Methods The normal and stripped stratum corneum skin of back and abdomen was used to study the transdermal absorption characteristic by Franz method. The transdermal absorption sample was taken at the preset sampling time point. The sample content was determined by HPLC. The cumulative transdermal amount of per unit area was fitted with sampling time point, the transdermal rate constant (J value) was selected as comparative standard. Results The transdermal rate constant (J) of skin with stratum corneum stripped increased obviously than that of the normal skin, so was the released rate constant. Conclusion The skin stratum corneum has an notable impact on transdermal absorption characteristic of Sinomenine Liposome Patch. When the skin of experimental animal being prepared, the integrality should be paid more attention.
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AIM: To develop the assay of curcumol in Baofukang Suppository by capillary GC. METHODS: GC Internal standard method was used to determine curcumol in Baofukang Suppository with capillary column. RESULTS: In the assay, curcumol and the internal standard are both well separated from other substances. A good linear relationship is obtained in the range of 0.1～0.5?g(r=0.9995). The RSD of precision, repeatability and recovery are all less than 3%. CONCLUSION: The method is accurate, reliable, and repeatable, suitable for the assay of the preparation and curcuma oil.
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Albendazole and mebendazole were comparatively evaluated in 22 adult patients for their in vivo effects on hookworm eggs. Both drugs were given 200mg twice daily for three consecutive days. Stool specimens wore collected before treatment and during the following five days. The Stool egg count was carried out by Stolls method and each specimen was also cultured by Hara-da-Mori technique for at least eight days. The mean pre-treatment percentage of incubated hookworm eggs that developed to larvae was 75.3% in albendazole group and 68.8% in mebendazole group respectively. One day following the initiation of treatment the mean percentage was remarkably reduced to 0.25% in albeadaole group and no eggs developed to larval stage beyond day 1. 0ne day and two days following the initiation of treatment the mean percentage was 16.23% and 23.13% respectively in mebendazole group. No eggs developed to larval stage on day 3 and thereafter. Albendazole seems to have better ovicidal effect than mebendazole.Albendazole and mebendazole were also comparatively evaluated in 123 adult patients with single or mixed infections of hookworm, ascarisis, and trichuris. A single dose of 400mg was used for both drugs. 2 and 4 weeks after treatment, their stools were examined by brine flotation technique. The hookworm eggs negative conversion ratss 2 weeks after treatment were 78.8% and 26.4% respectively in albeadazole and mebendazole group; while those 4 weeks after treatment were 74.1% and 25.5% respectivley. For ascariasis, the eggs negative conversion rates were 98.0% and 92.0% respectively. For trichuriasis, the negative conversion rates were only 20.6% and 27.7% respectively